Virus:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Multiple Displacement Amplification:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Recombinant:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Staining:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Labeling:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Electron Microscopy:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Bradford Assay:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
RNA Sequencing:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
Gene Expression:Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751 Article ll OPEN ACCESS
Article Title: De novo GTP synthesis is a metabolic vulnerability for the interception of brain metastases.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies IMPDH Antibody (F-6) Santa Cruz Cat#sc-166551; RRID:AB_2127354 mouse anti-GAPDH Abcam Cat#ab8245; RRID:AB_2107448 APC-conjugated anti-human TRA-1-85 (CD147) Miltenyi Biotec Cat#130-128-900; RRID:AB_2921968 HRP conjugated secondary anti mouse BioRad Cat#1706516; RRID:AB_11125547 Bacterial and virus strains NEB Stable Competent E. coli NEB C3040 Biological samples MDA-MB-231 ATCC Cat#HTB-26 Normal human astrocytes Lonza Bioscience Discontinued HEK293T ATCC Cat#CRL-11268 Chemicals, peptides, and recombinant proteins Mycophenlic acid Tocris Cat#1505 PrestoBlue Cell Viability Reagent ThermoFisher Scientific Cat#A13261 SYTOXTM Green Nucleic Acid Stain Invitrogen Cat#S7020 Incucyte Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling Sartorius Cat4717 7AAD Viability dye Beckman Coulter Cat#A07704 Liberase Blendzyme 3 Millipore Sigma Cat#5401119001 Heparin Solution Cat#07980 Human Recombinant Basic Fibroblast Growth Factor (bFGF) STEMCELL Technologies Cat#78003 Human Recombinant Epidermal Growth Factor (EGF) STEMCELL Technologies Cat#78006 Antibiotic-Antimycotic (100X) Wisent Bioproducts Cat#450-115-EL Lipofectamine 3000 ThermoFisher Cat#L3000075 Fetal Bovine Serum, heat inactivated (FBS) Wisent Cat#098-150 PBS ThermoFisher Cat#10010049 DMEM ThermoFisher Cat#11995073 EDTA Millipore Sigma Cat#20158 Guanine Sigma-Aldrich Cat#G11950 Paraformaldehyde Electron Microscopy Biosciences Cat#RT15700 D-firefly luciferin potassium salt Perkin Elmer Cat#122799 Critical commercial assays Bradford Assay BioRad Cat#5000112 Deposited data Lung-brain metastasis RNA seq data Gene Expression Omnibus GSE110495 Breast- and melanoma-brain metastasis RNA seq data Gene Expression Omnibus GSE220156 Metabolomics data FigShare https://doi.org/10.6084/m9.figshare.22246258 Experimental models: Cell lines Patient-dervied BM lines This manuscript N/A Neural Stem Cells This manuscript N/A CRUK0748, CRUK0733 Provided by Prof. Charles Swanton N/A (Continued on next page) Cell Reports Medicine 5, 101755, October 15, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Organisms/strains NOD-scid IL2Rgammanull (NSG) Mouse The Jackson Laboratory RRID:IMSR_JAX:005557 Recombinant DNA pMD2.G Addgene Cat#12259; RRID:Addgene_12259 psPAX2 Addgene Cat#12260; RRID:Addgene_12260 Toronto KnockOut (TKO) CRISPR Library - Version 3 Addgene Cat#:90294; RRID:Addgene_52961 Firefly Luciferase Addgene RRID:Addgene_118017 Software and algorithms Image Scope Aperio https://aperio-imagescope.software. informer.com Living Image Perkin Elmer https://www.perkinelmer.com/es/ lab-products-and-services/resourcesinvivo-imaging-software-downloads.html R The R project for Statistical Computing https://www.r-project.org/ GraphPad Prism Graph Pad https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ FlowJo v10.8 FlowJo LLC https://www.flowjo.com/ BioRender BioRender https://biorender.com/ ELDA: Extreme Limiting Dilution Analysis Walter+Eliza Hall Bioinformatics -Insitute of Medical Research https://bioinf.wehi.edu.au/software/elda/ Aperio ScanScope Leica Biosystems N/A FLUOstar Omega Fluorescence 556 Microplate reader BMG labtech N/A Other 10 mL Hamilton syringe Hamilton Cat#76350-01 NeuroCult NS-A Proliferation Kit Stem Cell Technologies Cat#05751
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